Genetic diversity of Colletotrichum lindemuthianum races based on ITS-rDNA regions
نویسندگان
چکیده
منابع مشابه
Sexual recombination in Colletotrichum lindemuthianum occurs on a fine scale.
Glomerella cingulata f. sp phaseoli is the sexual phase of the fungus Colletotrichum lindemuthianum, the causal agent of common bean anthracnose. This fungus is of great concern, because it causes large economic losses in common bean crops. RAPD markers of five populations of G. cingulata f. sp phaseoli from two Brazilian states were analyzed to determine if this population possesses the sexual...
متن کاملTranscriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem
Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illu...
متن کاملVegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil.
The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibility groups (VCGs) and by RAPD analysis. Two hundred and ninety-five ni...
متن کاملKinetic studies of the polygalacturonase enzyme from Colletotrichum lindemuthianum.
The intrinsic protein fluorescence of the polygalacturonase from Colletotrichium lindemuthianum was exploited in stopped-flow experiments aimed at elucidating the kinetic mechanism for this enzyme. Binding of the polymeric substrate polygalacturonic acid (PGA) essentially produced a triphasic fluorescence profile. There was an initial rapid quench in fluorescence, consistent with the rapid form...
متن کاملTemplate Preparation for Rapid Pcr in Colletotrichum Lindemuthianum
Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template....
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ژورنال
عنوان ژورنال: Agronomy Science and Biotechnology
سال: 2020
ISSN: 2359-1455
DOI: 10.33158/asb.r112.v6.2020